Characterization of PCR-ribotyping for Burkholderia (Pseudomonas) cepacia
نویسندگان
چکیده
منابع مشابه
Characterization of PCR-ribotyping for Burkholderia (Pseudomonas) cepacia.
Ribotyping, a method of genotyping bacterial isolates for epidemiologic study, uses rRNA as a probe to detect chromosomal restriction fragment length polymorphisms. Although ribotyping is accurate, its utility is limited by the labor and time necessary for Southern blot analysis. PCR-ribotyping uses PCR to amplify the 16S-23S intergenic spacer region of the bacterial rRNA operon. Length heterog...
متن کاملComparison of different PCR approaches for characterization of Burkholderia (Pseudomonas) cepacia isolates.
In this study, we evaluated three PCR methods for epidemiological typing of Burkholderia (Pseudomonas) cepacia--PCR-ribotyping, arbitrarily primed PCR (AP-PCR) and enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR)--and compared them with pulsed-field gel electrophoresis. The analysis was performed with 31 isolates of B. cepacia, comprising 23 epidemiologically unrelated is...
متن کاملDevelopment of a PCR probe test for identifying Pseudomonas aeruginosa and Pseudomonas (Burkholderia) cepacia.
AIMS To develop a system of species specific polymerase chain reaction (PCR) and DNA hybridisation based on 16s ribosomal RNA sequences for the identification of Pseudomonas aeruginosa and Pseudomonas (Burkholderia) cepacia in sputum from children with cystic fibrosis. METHODS Most of the 16s rRNA sequences from strains of Ps aeruginosa, Ps (Burkholderia) cepacia, and Ps putida were determine...
متن کاملDistinguishing species of the Burkholderia cepacia complex and Burkholderia gladioli by automated ribotyping.
Several species belonging to the genus Burkholderia are clinically relevant, opportunistic pathogens that inhabit major environmental reservoirs. Consequently, the availability of means for adequate identification and epidemiological characterization of individual environmental or clinical isolates is mandatory. In the present communication we describe the use of the Riboprinter microbial chara...
متن کاملInterspecies biofilms of Pseudomonas aeruginosa and Burkholderia cepacia.
The leading cause of morbidity and mortality in cystic fibrosis (CF) continues to be lung infections with Pseudomonas aeruginosa biofilms. Co-colonization of the lungs with P aeruginosa and Burkholderia cepacia can result in more severe pulmonary disease than P. aeruginosa alone. The interactions between P. aeruginosa biofilms and B. cepacia are not yet understood; one possible association bein...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1994
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.32.10.2422-2424.1994